<scp>Meta?Fish?Lib</scp> : A generalised, dynamic <scp>DNA</scp> reference library pipeline for metabarcoding of fishes

The accuracy and reliability of DNA metabarcoding analyses depend on the breadth quality reference libraries that underpin them. However, there are limited options available to obtain curate huge volumes sequence data public repositories such as NCBI BOLD. Here, we provide a pipeline download, clean annotate mitochondrial for given list fish species. Features this include (a) support multiple metabarcode markers; (b) searches species synonyms taxonomic name validation; (c) phylogeny assisted control identification removal misannotated sequences; (d) automatically generated coverage reports each new GenBank release update; (e) citable, versioned DOIs. As an example ready-to-use curated library marine freshwater fishes U.K. To augment environmental specifically, 241 MiFish-12S sequences 88 species, make primer sets useful sequencing these. This brings common fragment 93%, opening avenues scaling up across wide spatial gradients. Meta-Fish-Lib is hosted at https://github.com/genner-lab/meta-fish-lib.